Talk:Cell culture

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[Untitled]

I'm giving this article some attention, but welcome anyone else who would like to work on expanding it. Courtland 02:25, 2005 Mar 13 (UTC)

Article organization

current thought on organizing the article:

Introduction (present)
Key concepts (begun)
Uses of cell culture
Specific examples
Notes & references (begun)
External links to methods and manuals
Related topics

I'm thinking that all types of cell culture could be addressed in a general sense, with specific articles spinning off for things like insect cell culture, plant tissue culture, culture of anaerobic bacterial pathogens, viral culture, etc. Courtland 02:29, 2005 Mar 13 (UTC)

I don't think that there needs to be much mention of microbial culturing. Make a link to microbiology, fermentation etc. In my experience cell culture in it's modern sense means, to the laboratory scientist, mainly animal and plant cell culture. Microbial cell culture is a term one hardly ever hears.--Alun 06:04, 11 May 2005 (UTC)[reply]

NO LEGAL ISSUES?

Interesting that article ignores the many legal issues re cell cultures. a. Are donors informed that their cells re being used? b. Are donors paid for their cells? c. Who owns the patents, trademarks? d. Who monitors the legal ramifications of cell cultture businesses in order to protect patients, the public? — Preceding unsigned comment added by 2604:2000:DDC7:1700:1180:C261:C499:5D79 (talk) 04:50, 5 December 2017 (UTC)[reply]

. — Preceding unsigned comment added by Abcdefghikjlmnop (talk • contribs) 21:43, 30 May 2020 (UTC)[reply]

Morpholino

Sorry for my ignorance, but what is the relevance of this section to "cell culture"? Kaisershatner 18:32, 18 April 2006 (UTC).[reply]

"Preclinical research with Morpholino antisense oligos has shown efficacy of the antisense against influenza in cell cultures. Cultures of African green monkey kidney cells (vero cells) were pretreated with Morpholino antisense oligos conjugated with arginine-rich peptides to enhance penetration of the oligos into the cytosol. Targeting translation-blocking Morpholinos against the nucleoprotein 1 mRNA or one of the polymerase protein mRNAs caused 2-3 log10 reductions in influenzavirus titer three days post-infection. When Morpholinos were administered post-infection, less antiviral activity was measured. ^[1]

AVI BioPharma reports that when tested against several influenza strains by several independent laboratories, Morpholino oligos have suppressed viral replication and in one cse both replication and transcription were repressed. Co-administration of several Morpholino sequences caused as much as eightfold improvement in antiviral activity..^[2]

Can you explain why it doesn't belong in cell culture? Don't the two paragraphs talk about cell culture? If you are saying other stuff is more directly relevant, then my response is, well add that more directly relevent stuff and then the less directly relevant stuff can be removed at that time. If you are saying, no it really isn't even indirectly related, then you must know more about this than I do (which wouldn't be hard, I know very little about it); in which case we just keep it removed. WAS 4.250 19:31, 18 April 2006 (UTC)[reply]

op.cit

Shouldn't "op.cit" contain more information? WAS 4.250 19:23, 18 April 2006 (UTC)[reply]

Cell culture and Tissue culture fusion

For something that has so many applications in agriculture, medicine, and basic research both articles are extremely weak. The tissue culture should have the history and animal-plant distinctions and organ,tissue, cell distinction. I can see the cell culture needs to be its own article but is it at that stage presently or should it be fused with the weaker Tissue culture? The history of tissue culture could be a distinct article with the history of methodologies in culture, synchronizing cells, labeling cells, heterokaryons, hybridomas, suspension, substrates, transformation, contact inhibition, Hayflick senescence and now telomeres, SV-40 virus, etc. GetAgrippa 19:05, 26 January 2007 (UTC)[reply]

Tempted to vandalise

I am tempted to add at the top:

Maybe on 1 april next year... --Squidonius (talk) 15:21, 9 May 2008 (UTC)[reply]

Insect cell culture

Culture of non-mammalian cells needs a section on insect cell culture. I'm not an expert, and was actually looking to see if there was anything here on the topic. In my very limited understanding, I gather that people sometimes use insect cells in applications when mammalian cells might introduce unwanted background (because there is so much difference between insects and mammals). Can someone else comment or make an appropriate contribution to the article? Lcwilsie (talk) 16:09, 9 May 2008 (UTC)[reply]

cell line lists

There are two lists of common cell lines at the end of this article. Perhaps they should be combined into one? 142.103.207.10 (talk) 22:31, 14 May 2008 (UTC)[reply]

Absolutely. I like the table format, in that it is sort-able. However, since the table isn't comprehensive (and would be ridiculously long if it were), does the list add any value? How do we decide which cell lines to include? Is there an external link somewhere with a comprehensive list? Should there simply be some representative examples cited in the text (such as, "Cell lines have been generated from many tissues and species such as _______")? Lcwilsie (talk) 13:31, 21 May 2008 (UTC)[reply]

partially Concur I added 2 lines as it took me 2 hours to find out what they actually stand for (Unfortuantelly it is nice trivial information that is getting lost). People that check the Methods pages on wikipedia tend to be people in research that are looking up facts like "how does it work" or what does that stand for. So I would opt to merge and keep the table, maybe on a separte page so less offensive to the eye? although the morphology section may be incorrect in terminology but I cannot find the right ref.--Squidonius (talk) 21:13, 21 May 2008 (UTC)[reply]

Which information are you having difficulty finding? I find all of the information we have on the tables at ATCC or the relevent supplier's page. I concur with moving the list to a separate page, but still find the inclusion of the list unnecessary. I could add all of the cell lines I work with (~20), but would that be useful to someone in a completely different field? Probably not. It needs to be 100% inclusive or absolutely minimal. Lcwilsie (talk) 17:56, 26 August 2008 (UTC)[reply]

I'd suggest the latter as presumably an entry in the list is the first step towards individual articles for many of the cell lines. I have done some work adding and merging the two lists but am I alone in thinking a culture conditions (media+supplements) column might be worthwhile? This could be achieved by merging the largely duplicative/redundant tissue of origin and morphology sections —Preceding unsigned comment added by Yvaud (talk • contribs) 13:35, 2 September 2008 (UTC)[reply]

Media to use would be a valid column, but the info can be found on the ATCC webpage (although everyone knowns "someone" who has failed at growing cells in the wrong media as they were given the cells by the former lab neighbour of their supervisor when he was a student, but that is just internal misinformation that cannot be solved by wikipedia). As mentioned I like this table as it says what the acronyms mean. The fact that it is not complete is not an issue: people add their cell lines, not ones lost in the archives, so is actually a sample of cell lines currently in use. --Squidonius (talk) 06:55, 22 April 2010 (UTC)[reply]

I have a lot of opinions about this. :) I like the table, myself. I worked with many of these cell lines in college and it is a nice quick reference. I suggest removing the common cell lines section above it and integrating any not present. The meaning column is very useful but the title of the column 'Meaning', may not be. The links column should be removed entirely. Most of the links do not even work anymore. Perhaps something more useful would be any database catalogue numbers associated with the lines similar to the CAS registry numbers and EC numbers for each of the linked databases. Also, we can decide what makes it on this list by possibly a threshold number of journal articles referencing the cell lines. If more than one university uses a cell line or the cell line is used in multiple labs by multiple investigators, it should be included on this list. If the list gets too large, we can move it to its own page but as it is, it is not too long yet. And finally about the proposed media column... That would not be very useful just because most labs have their own modified versions of media to use for the same cell lines and the suggested media is always readily available at the source website we buy from. Ataylor18 (talk) 20:07, 1 April 2015 (UTC)[reply]

MDA-MB-435

The melanomic origin of MDA-MB-435 does not seem to be disputed, but firmly established. Even the link in the article states it as a matter of fact (and rightfully so). Because of the importance of the topic (30 years of breastcancer research need to be reviewed) an expert should take a look at it. 217.231.48.197 (talk) 17:27, 30 October 2008 (UTC).[reply]

Ma-Mel / MEL CELL LINES

Does anyone know if the Ma-Mel cell lines and Mel (e.g Mel 624) cell lines are one and the same?Yvaud (talk) —Preceding undated comment added 02:41, 17 January 2010 (UTC).[reply]

Cell lines vs. cell culture

I think the article could spell out the difference (if any?) between cell line and cell culture as cell line redirects here. Throughout the article the two are used somewhat interchangeably which I find undesirable since the terms -- at least in my head -- are not completely the same. Can any expert elaborate? Thanks. Bilgrau (talk) 09:44, 1 February 2012 (UTC)[reply]

@Bilgrau: - A cell line is something you grow with cell culture techniques, in the same way a fruit tree is something you grow with gardening techniques. NickCT (talk) 16:28, 9 December 2014 (UTC)[reply]

ثقافة النباتية خلية معزول

ثقافة النباتية خلية معزول — Preceding unsigned comment added by 197.207.147.196 (talk) 11:10, 20 October 2012 (UTC)[reply]

Cell_culture#List_of_cell_lines duplication error?

Hmmmmm.... Maybe I'm missing something here, but it appears as though there been some kind of weird duplication error in the list of cell lines. I'm going to attempt to address it. NickCT (talk) 16:12, 9 December 2014 (UTC)[reply]

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Cell Culture Conditions

Including the reference for hPL vs. FBS. Also, the general culturing parameters of 37C and 5% CO2 should be labelled as typical as some cell lines like MDA-kb2 do not need CO2 at all.

^[3]

BBCBAB (talk) 15:14, 12 August 2017 (UTC)BBCBAB (talk) 21:29, 14 August 2017 (UTC)SJH[reply]

Refactoring of the cell line lists

Please see (and add to) Wikipedia Talk:WikiProject Molecular and Cell Biology#Refactoring of the cell line lists if you are interested in this. Tomdo08 (talk) 20:25, 21 January 2018 (UTC)[reply]

References

This section is not about the references section in the subject page: There are references in this talk page; without putting the reflist into a separate chapter, it looks like they belong to the random chapter which happens to be the last.

^ (Frederick G. Hayden, MD, Influenza in the United States and Around the World, http://www.medscape.com/viewarticle/495517, see "novel therapeutics" section)(Ge Q, Stein D, Kroeker A, Iversen P, Chen J. Inhibition of influenza A virus production in vero cells with morpholino oligomers. Program and abstracts of the 44th Interscience Conference of Antimicrobial Agents and Chemotherapy; October 30-November 2, 2004; Washington, DC. Abstract V-1268.)
^ AVI BioPharma
^ Hemeda, H., Giebel, B., Wagner, W. (16Feb2014) Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells Cytotherapy p170-180 issue 2 doi.10.1016

Weird sentence in 'Applications of cell culture'

I'm not sure, what it should say and English isn't my naive language, so I didn't dare to edit it, but does the following paragraph make sense?

"An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants, use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy observation is one of its applications. It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the process." — Preceding unsigned comment added by Carroll D. (talk • contribs) 23:22, 22 November 2021 (UTC)[reply]

Wiki Education assignment: Molecular Genetics

This article was the subject of a Wiki Education Foundation-supported course assignment, between 22 August 2022 and 9 December 2022. Further details are available on the course page. Student editor(s): Ayda P (article contribs).

— Assignment last updated by Ayda P (talk) 03:57, 12 September 2022 (UTC) I have combined Tissue culture and Cell culture articles and added few sentences and cited the papers I used to do the edits — Preceding unsigned comment added by Ayda P (talk • contribs) 19:50, 17 September 2022 (UTC)[reply]

[1] (Frederick G. Hayden, MD, Influenza in the United States and Around the World, http://www.medscape.com/viewarticle/495517, see "novel therapeutics" section)(Ge Q, Stein D, Kroeker A, Iversen P, Chen J. Inhibition of influenza A virus production in vero cells with morpholino oligomers. Program and abstracts of the 44th Interscience Conference of Antimicrobial Agents and Chemotherapy; October 30-November 2, 2004; Washington, DC. Abstract V-1268.)

[2] AVI BioPharma

[3] Hemeda, H., Giebel, B., Wagner, W. (16Feb2014) Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells Cytotherapy p170-180 issue 2 doi.10.1016

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